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5 thoradh

  2. PRODUCTION, TECHNOLOGY AND RESEARCH|technology and technical regulations|biotechnology · SCIENCE|natural and applied sciences|life sciences|biology

    #664893

    IATE #3593059
    eangú Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    nicking | to nick
    en
    Sainmhíniú cutting only the one of the two strands of DNA Tagairt "COM-Terminology Coordination, based on:- EFSA Panel on Genetically modified organisms (GMO); 'Scientific opinion addressing the safety assessment of plants developed using Zinc Finger Nuclease 3 and other Site-Directed Nucleases with similar function' (2.3.2021). EFSA Journal 2012;10(10):2943. [31 pp.] doi:10.2903/j.efsa.2012.2943. Available online: www.efsa.europa.eu/efsajourna- 'Nicking Endonucleases: The Discovery and Engineering of Restriction Enzyme Variants' (3.3.2021). New England BioLabs"
  3. SCIENCE|natural and applied sciences|life sciences|biology|genetics

    #666074

    IATE #3619824
    imoibriú aimpliúcháin einsíme eangúcháin Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    NEAR Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    nicking enzyme amplification reaction | NEAR
    en
    Sainmhíniú isothermal amplification method where a set of target-specific primers, a nicking endonuclease, and a strand displacing DNA polymerase are used for isothermal unwinding and subsequent generation and amplification of these small DNA or RNA fragments Tagairt "COM-PT based on: Dunbar, S., Das, S., 'Amplification chemistries in clinical virology' (1.9.2021), Journal of Clinical Virology, 019 Jun; 115: 18–31."
  4. SCIENCE|natural and applied sciences|life sciences|biology

    #617650

    IATE #1073606
    aistriú eanga Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    Nick-Translation
    de
    nick translation
    en
    Sainmhíniú tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in fluorescent in situ hybridization (FISH) or blotting techniques as well as for radiolabeling Tagairt "Wikipedia > Nick translation (24.3.2021)"
    Nóta Nick translation was the first method devised for the in vitro labeling of DNA. During the reaction the DNA to be labeled is nicked by DNase I yielding a free 3′ hydroxyl end. DNA polymerase I then adds a new nucleotide to this end. The 5′–3′ exonuclease activity of the polymerase then moves the “nick” along the strand in the 3′ direction.
    translation de coupure | translation d'entaille | translation de brèche | translation de cassure | translation de césure
    fr
    Sainmhíniú Technique utilisée en biologie moléculaire, permettant de marquer par des radio-isotopes un ADN bicaténaire. L'ADN polymérase 1(d'E. coli) se fixe sur une coupure (un nick) d'un brin, en enlevant les nucléotides à partir de l'extrémité 5'(grâce à son activité exonucléasique 5'3')et les remplace simultanément: il y a copie du brin complémentaire à l'extrémité 3'(grâce à son activité polymérasique 5'3') Tagairt JO F 18.8.87;ADEBIO,p.362
  5. PRODUCTION, TECHNOLOGY AND RESEARCH|technology and technical regulations|biotechnology · SCIENCE|natural and applied sciences|physical sciences|chemistry|biochemistry|enzyme

    #634482

    IATE #1685063
    einsím eangúcháin Tagairt "Faofa ag an gCoiste Téarmaíochta, www.tearma.ie Faomhadh an téarma seo mar chuid de Thionscadal Lex"
    ga
    ion-núicléáis eangúcháin Tagairt "Faofa ag an gCoiste Téarmaíochta, www.tearma.ie Faomhadh an téarma seo mar chuid de Thionscadal Lex"
    ga
    Nick-Translation
    de
    nicking enzyme | nicking endonuclease | NEase | nickase | single-strand specific DNA and RNA endonuclease | single-strand nicking endonuclease | single-strand nickase
    en
    Sainmhíniú new type of enzyme that recognises a short specific DNA sequence and cleaves DNA at a fixed position relatively to the recognition sequence (like restriction endonucleases) but cleaves only one predetermined DNA strand (unlike restriction endonucleases) Tagairt "COM-Terminology Coordination, based on: L. A. Zheleznaya, G. S. Kachalova, R. I. Artyukh, A. K. Yunusova, T. A. Perevyazova, and N. I. Matvienko, 'Nicking Endonucleases' (2.3.2021), Biochemistry (Moscow), 2009, Vol. 74, No. 13, pp. 14571466. © Pleiades Publishing, Ltd., 2009."
    entaillase
    fr
    Sainmhíniú endonucléase capable de produire une entaille dans une molécule d'ADN double-brin Tagairt Dict.de génétique,CILF,1991