Gaois

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  1. SOCIAL QUESTIONS|health|medical science|toxicology

    #680246

    IATE #1594429
    cóilíneacht cúlchlaochlóra Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    Revertanten-Kolonie | Revertantenkolonie
    de
    revertant colony
    en
    Definition "the Ames test [ IATE:3526642 ] is a bioassay for testing the mutagenic potential of chemicals on the basis of the frequency of back (reverse) mutations [ IATE:1351112 ] in histidine auxotrophic mutants of the pathogenic bacterium Salmonella typhimurium (i.e. these bacteria have been mutated such that they are no longer able to synthesize the amino acid histidine themselves, so they need it in the medium). At the start of the experiment, a certain amount of histidine auxotrophs are inoculated on an agar plate containing a rich medium with, however, a little bit of histidine and the chemical to be tested. (Usually, there are several plates with different concentrations of chemical.) The inoculated bacteria grow and divide, until the histidine is exhausted. So each produces a micro-colony, usually consisting of some 100 bacteria. If one or several bacteria have been permanently converted to the prototrophic state (so that the cells can grow on a histidine-free medium), however, its colony will continue to grow and will become visible with the naked eye. It is then called a revertant colony" Reference "COM-Terminology Coordination, based on:Faculty of Earth and Life Sciences. The Ames test http://www.bio.vu.nl/thb/course/tb/exam/exam/node12.html [21.9.2010]"