SCIENCE|natural and applied sciences|life sciences
- tíopa bíge Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
- ga
- PFGE type | Pulsed-field gel electrophoresis typing | PFGE typing | pulse type
- en
- Definition "genotype1 determined with pulsed-field gel electrophoresis2 1 genotype [ IATE:1622107] 2 pulsed-field gel electrophoresis [ IATE:1147442 ]" Reference "COM-Terminology Coordination, based on:Applied Maths. Pulsed-field gel electrophoresis (PFGE) typing, http://www.applied-maths.com/applications/pulsed-field-gel-electrophoresis-pfge-typing [29.7.2014]"
- Comment Pulsed-field gel electrophoresis (PFGE) is a highly discriminative molecular typing technique that is used in epidemiological studies worldwide. PFGE is based upon the variable migration of large DNA restriction fragments in an electrical field of alternating polarity. By comparing the fingerprints of any two isolates, one can investigate if they belong to the same strain (i.e. the two isolates are clonal) or if they are genetically unrelated. The implementation of PFGE in international surveillance networks, such as PulseNet, and the availability of standardized protocols for important foodborne pathogens (E. coli, Listeria, Campylobacter, …) have facilitated the adoption of this technique. Although more recent typing methods are available, PFGE is still the gold standard in many national and international surveillance programs.