Gaois

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  1. SCIENCE|natural and applied sciences|life sciences|biology|genetics

    #574269

    IATE #3510195
    ISP-CT neadaithe Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    imoibriú slabhrúil polaiméaráise neadaithe cúl-trascrioptáise Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    mehrstufige RT-PCR
    de
    nested set RT-PCR | nested RT-PCR | reverse-transcription nested polymerase chain reaction
    en
    Definition technique based on RT-PCR1 where a sample of the product from a primary RT-PCR is taken and used as a template for a secondary round of PCR2 amplification3 in which a different set of primers4 (called "nested" primers because they are internal to the primers used in the primary PCR) is employed to avoid further amplification of primer-dimer5 artifacts or nonspecific products generated in the primary PCR Reference COM-Terminology Coordination, based on: Goode T. et al. (2002) Nested RT-PCR (12.4.2019). In: O’Connell J. (eds) RT-PCR Protocols. Methods in Molecular Biology, vol 193. Humana Press
    Comment 1 RT-PCR: 19046112 PCR: IATE:1431892<;;;; amplification: 1473998 4 primers:10733775 primer-dimer artefacts: 3580676