Gaois

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3 results

  1. PRODUCTION, TECHNOLOGY AND RESEARCH|technology and technical regulations|biotechnology

    #620633

    IATE #1142265
    tras-scríobh <i>in vitro</i> Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    in vitro-Transcription
    de
    Definition Verfahren, bei dem mRNA zellfrei, d.h.unter Verwendung entsprechend aufbereiteter Zellextrakte, in vitro synthetisiert wird. Reference Ibelgaufts H.,Gentechnologie von A bis Z,VCH Verlag
    in vitro transcription
    en
    Definition laboratory procedure that allows for template-directed synthesis of RNA molecules of any sequence from short oligonucleotides to those of several kilobases in μg to mg quantities based on engineering of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using the corresponding RNA polymerase Reference "Beckert B., Masquida B. (2011) 'Synthesis of RNA by In Vitro Transcription' (3.3.2021). In: Nielsen H. (eds) RNA. Methods in Molecular Biology (Methods and Protocols), vol 703. Humana Press."
  2. SCIENCE|natural and applied sciences|life sciences

    #628995

    IATE #1464555
    cúl-tras-scríobh Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    reverse Transkription
    de
    reverse transcription
    en
    Definition "synthesis of single-stranded DNA (complementary DNA, or cDNA)1 using single-stranded RNA as a template, mediated by reverse transcriptases (RTs)21cDNA [ IATE:1073342 ]2reverse transcriptase [ IATE:1311710 ]" Reference "Life Technologies. Reverse Transcription, https://www.lifetechnologies.com/lu/en/home/life-science/pcr/reverse-transcription.html [7.8.32015]"
    Comment "The cDNA can be used as a template for amplification by PCR1 or to generate a cDNA library.Choosing the correct reverse transcriptase for cDNA synthesis is critical to obtaining high yields of quality, full-length cDNA with full gene representation.1PCR [ IATE:1431892 ]"
    transcription inverse
    fr
  3. SCIENCE|natural and applied sciences|life sciences|biology

    #617574

    IATE #1073404
    tras-scríobh Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    Transkription
    de
    Definition "Synthese einer komplementären, einzelsträngigen m-,t- und ribosomalen RNS, die jeweils dem 2. DNS-Strang gleicht, d.h. ""Umschreibung"" des DNS- in einen RNS-Text. Das Umschreiben der Erbinformation von der DNA, in der sie als Abfolge von Nucleotiden gespeichert ist, in RNA" Reference Roche Lexikon Medizin & Buddecke,p.581;Vogel S.,Lexicon Gentechnik,Rowohlt Verlag
    Comment Bei der Transcription werden in einem Teilabschnitt der DNA die beiden Stränge des Moleküls getrennt. An einem der beiden Stränge (der Matrize) wird durch Basenpaarung ein RNA-Strang aufgebaut, dessen Sequenz komplementär zum DNA-Strang ist. Diese RNA kann entweder als ribosomale RNA oder transfer-RNA selbst Aufgaben in der Zelle übernehmen, oder sie enthält als Boten-RNA die Information, die sie zum Aufbau eines Proteins benötigt wird.
    transcription
    en
    Definition "process by which the genetic information encoded in the gene is copied with RNA polymerase into a complementary sequence of ribonucleotides known as mRNA" Reference "COM-EN, based on:Wikipedia > Gene (26.5.2020)"
    transcription
    fr
    Definition processus permettant la copie de l'ADN en ARN Reference "CNRS. Glossaire http://www.cnrs.fr/cw/dossiers/doscel/glossaire/global.htm [15.3.2011]"