Gaois

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6 results

  1. SCIENCE|natural and applied sciences|life sciences

    #628995

    IATE #1464555
    cúl-tras-scríobh Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    reverse Transkription
    de
    reverse transcription
    en
    Definition "synthesis of single-stranded DNA (complementary DNA, or cDNA)1 using single-stranded RNA as a template, mediated by reverse transcriptases (RTs)21cDNA [ IATE:1073342 ]2reverse transcriptase [ IATE:1311710 ]" Reference "Life Technologies. Reverse Transcription, https://www.lifetechnologies.com/lu/en/home/life-science/pcr/reverse-transcription.html [7.8.32015]"
    Comment "The cDNA can be used as a template for amplification by PCR1 or to generate a cDNA library.Choosing the correct reverse transcriptase for cDNA synthesis is critical to obtaining high yields of quality, full-length cDNA with full gene representation.1PCR [ IATE:1431892 ]"
    transcription inverse
    fr
  2. SCIENCE|natural and applied sciences|life sciences|biology|genetics

    #683773

    IATE #3563577
    imoibriú slabhrúil polaiméaráise cúl-trascrioptáise cainníochtúil fíor-ama Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    ISP-CT cainníochtúil fíor-ama Reference Téarmeolaithe COM-GA
    ga
    quantitative Echtzeit-RT-PCR | Echtzeit-Reverse-Transkriptase-Polymerase-Kettenreaktion
    de
    real-time reverse transcription PCR | real-time reverse-transcription polymerase chain reaction | real-time RT-PCR | RT-qPCR | real-time reverse transcriptase polymerase chain reaction | quantitative RT-PCR | quantitative real-time RT-PCR | real-time quantitative reverse transcription polymerase chain reaction | qRT-PCR | RT-(q)PCR
    en
    Definition "in vitro1method for enzymatically amplifying defined sequences of RNA2 1 RNA (related) [ IATE:1073359 ] 2 in vitro (related) [ IATE:1364825 ]" Reference "Jelena Brkic. Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Analysis. BIOL5081. York University, http://www.yorku.ca/shore/Grad%20stats/qRT%20PCR%20posting.pptx [18.12.2014]"
    réaction en chaîne par polymérase en temps réel après transcription inverse | RT-PCR quantitative | qRT-PCR
    fr
    Definition technique ayant pour but de quantifier un type d’ARN initialement présent dans un échantillon par la réalisation d'une PCR (réaction en chaîne par polymérase) à partir d’un échantillon d’ARN d'abord rétrotranscrit grâce à une enzyme appelée transcriptase inverse, qui permet la synthèse de l’ADN complémentaire (ADNc) utilisé pour la PCR Reference "COM-FR d'après le site de Futura-sciences > santé > dictionnaire > RT-PCR, http://www.futura-sciences.com/magazines/sante/infos/dico/d/medecine-rt-pcr-13491/ [23.9.2015]"
  3. SCIENCE|natural and applied sciences|life sciences|biology

    #686166

    IATE #3593280
    aimpliú isiteirmeach cúl-trascrioptáise trí mheán lúb Reference "Faofa ag an gCoiste Téarmaíochta, www.tearma.ie Faomhadh an téarma seo mar chuid de Thionscadal Lex"
    ga
    reverse transcription loop-mediated isothermal amplification | RT-LAMP
    en
    Definition one-step nucleic acid amplification method to multiply specific sequences of RNA Reference "Wikipedia > Reverse Transcription Loop-mediated Isothermal Amplification (16.3.2021)"
    Comment "It combines LAMP DNA-detection with reverse transcription, making cDNA from RNA before running the reaction. RT-LAMP does not require thermal cycles (unlike PCR) and is performed at a constant temperature between 60 and 65 °C.RT-LAMP is used in the detection of RNA viruses (Groups II, IV, and V on the Baltimore Virus Classification system), such as the SARS-CoV-2 virus and the Ebola virus."
  4. SCIENCE|natural and applied sciences|life sciences|biology|genetics

    #681277

    IATE #1904611
    imoibriú slabhrúil polaiméaráise cúl-trascrioptáise Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    ISP cúl-trascrioptáise Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    Umkehrtranskriptase-PCR | RT-PCR | Reverse-Transkriptase-Polymerase-Kettenreaktion
    de
    reverse transcriptase polymerase chain reaction | reverse transcription-polymerase chain reaction | reverse transcriptase-PCR | RT-PCR
    en
    Definition "laboratory technique combining reverse transcription [ 1464555 ] of RNA into DNA and amplification of specific DNA targets using polymerase chain reaction (PCR) [ 1431892 ]" Reference "- 'Reverse transcription polymerase chain reaction' (21.5.2019), Wikipedia."
    transcription inverse couplée à une réaction d’amplification en chaîne par polymérase
    fr
  5. SCIENCE|natural and applied sciences|life sciences|biology|genetics

    #682222

    IATE #3510195
    ISP-CT neadaithe Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    imoibriú slabhrúil polaiméaráise neadaithe cúl-trascrioptáise Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    mehrstufige RT-PCR
    de
    nested RT-PCR | nested set RT-PCR | reverse-transcription nested polymerase chain reaction
    en
    Definition "technique based on RT-PCR1 where a sample of the product from a primary RT-PCR is taken and used as a template for a secondary round of PCR2 amplification3 in which a different set of primers4 (called ""nested"" primers because they are internal to the primers used in the primary PCR) is employed to avoid further amplification of primer-dimer5 artifacts or nonspecific products generated in the primary PCR" Reference "COM-Terminology Coordination, based on: Goode T. et al. (2002) Nested RT-PCR (12.4.2019). In: O’Connell J. (eds) RT-PCR Protocols. Methods in Molecular Biology, vol 193. Humana Press"
    Comment "1 RT-PCR: 19046112 PCR: IATE:1431892<;;;;;;;;; amplification: 1473998 4 primers:10733775 primer-dimer artefacts: 3580676 "
  6. SCIENCE|natural and applied sciences|life sciences|biology

    #665202

    IATE #3598788
    aimpliúchán polaiméaráise athchuingráis cúl-trascrioptáise Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    RT-RPA Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    RT-RPA
    de
    reverse-transcription recombinase polymerase amplification | RT-RPA
    en
    Definition "recombinase polymerase amplification system where reverse transcriptase is also added in order to detect RNA" Reference "COM-Terminology Coordination, based on: Matthew D. Moore and Lee-Ann Jaykus, 'Recombinase polymerase amplification: a promising point-of-care detection method for enteric viruses' (1.4.2022). Future Virology, 2017; 12(8): 421-429"