PRODUCTION, TECHNOLOGY AND RESEARCH|technology and technical regulations|biotechnology · SCIENCE|natural and applied sciences|life sciences|biology
- clónáil chiumhaismhaol Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
- ga
- blunt-end cloning
- en
- Sainmhíniú "cloning of DNA fragments containing no unpaired bases at the 5’ and 3’ prime ends (i.e. blunt ends) into linearised plasmid vectors" Tagairt "COM-Terminology Coordination, based on: New England Biolabs, 'A Beginner’s Guide to How Blunt-End Cloning Works' (25.3.2021)"
- Nóta To obtain the maximum number of “correct” ligation products when cloning blunt-ended target fragments, the two components of DNA in the ligation reaction must be present at an appropriate ratio. If the molar ratio of plasmid vector to target DNA is too high, then the ligation reaction may generate an undesirable number of circular empty plasmids, both monomeric and polymeric; if too low, the ligation reaction may generate an excess of linear and circular homopolymers and heteropolymers of varying sizes, orientations, and compositions. For this reason, the orientation of the foreign DNA and the number of inserts in each recombinant clone must always be validated by restriction endonuclease mapping or some other means.