Gaois

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  1. SCIENCE|natural and applied sciences|life sciences

    #629193

    IATE #1474025
    bearna Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    Gap
    de
    Definition Lücke oder Leerstelle in einer Sequenz, insbesondere beim Sequenzalignment Reference "Wikipedia, s. v. ""Gap (Bioinformatik)"", http://de.wikipedia.org/wiki/Gap_(Bioinformatik) (4.4.2012)"
    Comment Gap bedeutet, dass an der entsprechenden Stelle in einer verwandten Sequenz ein weiteres Element steht
    gap
    en
    Definition maximal consecutive run of spaces in a single string of a given alignment Reference "European Bioinformatics Institute. Help - About Gaps In Sequence Alignments http://www.ebi.ac.uk/help/gaps.html [26.3.2012]"
    Comment It corresponds to an atomic insertion or deletion of a substring.Causes of gaps:- A single mutation can create a gap (very common).- Unequal crossover in meiosis can lead to insertion or deletion of strings of bases.- DNA slippage in the replication procedure can result in the repetition of a string.- Retrovirus insertions.- Translocations of DNA between chromosomes.
    brèche | espace vide
    fr
    Definition absence d'un ou plusieurs nucléotides dans un des deux brins de l'ADN Reference arrêté du 14/09/90 relatif à la terminologie du génie génétique,JORF 26/09/90
  2. SCIENCE|natural and applied sciences|life sciences|biology

    #617650

    IATE #1073606
    aistriú eanga Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    Nick-Translation
    de
    nick translation
    en
    Definition tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in fluorescent in situ hybridization (FISH) or blotting techniques as well as for radiolabeling Reference "Wikipedia > Nick translation (24.3.2021)"
    Comment Nick translation was the first method devised for the in vitro labeling of DNA. During the reaction the DNA to be labeled is nicked by DNase I yielding a free 3′ hydroxyl end. DNA polymerase I then adds a new nucleotide to this end. The 5′–3′ exonuclease activity of the polymerase then moves the “nick” along the strand in the 3′ direction.
    translation de coupure | translation d'entaille | translation de brèche | translation de cassure | translation de césure
    fr
    Definition Technique utilisée en biologie moléculaire, permettant de marquer par des radio-isotopes un ADN bicaténaire. L'ADN polymérase 1(d'E. coli) se fixe sur une coupure (un nick) d'un brin, en enlevant les nucléotides à partir de l'extrémité 5'(grâce à son activité exonucléasique 5'3')et les remplace simultanément: il y a copie du brin complémentaire à l'extrémité 3'(grâce à son activité polymérasique 5'3') Reference JO F 18.8.87;ADEBIO,p.362