SCIENCE|natural and applied sciences|physical sciences|chemistry|analytical chemistry
- éadáil neamhspleách ar shonraí Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
- ga
- Comhthéacs 'Nuair a dhéantar cinneadh mais-speictriméadrach trí bhloghadh tar éis máthair-ian a roghnú, déantar máthair-ian a roghnú de réir taifeach aonaid maise nó níos fearr. Is éard a bheidh sa mháthair-ian a roghnófar an t-ian móilíneach, aduchtanna saintréitheacha den ian móilíneach, mac-iain shaintréitheacha nó ceann dá n-iain iseatóip. I gcás ina ngabhann ionú maisroghnúcháin níos mó ná aon Dalton amháin le máthair-iain a roghnú (e.g. i gcás Éadáil Neamhspleách ar Shonraí) meastar gur anailís dheimhnitheach lánscanta í an teicníc.' Tagairt "Rialachán Cur Chun Feidhme (AE) 2021/808 maidir le modhanna anailíse a fheidhmiú i leith iarmhair substaintí atá gníomhach ó thaobh na cógaseolaíochta de agus a úsáidtear i mbia-ainmhithe agus maidir torthaí a léirmhíniú agus maidir leis na modhanna atá le húsáid le haghaidh samplála agus lena n-aisghairtear Cinneadh 2002/657/CE agus Cinneadh 98/179/CE, CELEX:32021R0808/GA"
- data-independent acquisition | DIA
- en
- Sainmhíniú "method of identification and/or quantification due to the continuous collection of fragment-ion spectra over a preselected precursor m/z range" Tagairt "COM-Terminology Coordination, based on: Heaven MR, Funk AJ, Cobbs AL, et al., 'Systematic evaluation of data-independent acquisition for sensitive and reproducible proteomics-a prototype design for a single injection assay' (12.2.2021), Journal of Mass Spectrometry, 2016;51(1):1-11. doi:10.1002/jms.3716"
- Nóta Multiple variations of the DIA approach have been applied; for a review see Tate et al.[17] For instance, in the MSE method,[12] high and low collision energy is used to fragment all precursors simultaneously from 400–1200 m/z. Whereas, in the sequential windowed acquisition of all theoretical fragment-ions (SWATH)[13] procedure, 26 m/z windows are stepped through every ~3 seconds covering the 400–1200 m/z precursor range in a single analysis. Other methods such as precursor acquisition independent from ion count (PAcIFIC)[14] use isolation windows as small as 2.5 m/z that provide ideal sensitivity and specificity, but require many analyses to assay the 400–1400 precursor m/z range (e.g., in each sample injection a 15 m/z precursor range is analyzed). Although these extreme differences in isolation windows sizes have been applied from 2.5 to 800 m/z in the PAcIFIC and MSE methods, respectively, systematic evaluation of this important parameter in various DIA methods and the precursor m/z ranges analyzed have not been reported.