Gaois

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  1. SCIENCE|natural and applied sciences|life sciences|biology|genetics
    ISP Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    imoibriú slabhrúil polaiméaráise neadaithe Reference Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    verschachtelte PCR | verschachtelte Polymerase-Kettenreaktion
    de
    nested polymerase chain reaction | nested PCR | nPCR
    en
    Definition "method of polymerase chain reaction (PCR) which uses two pairs of PCR primers for a single locus, the first pair amplifying the locus as seen in any PCR experiment, while the second pair of primers (nested primers) binds within the first PCR product and produces a second PCR product that will be shorter than the first one" Reference "COM-Terminology Coordination, based on:Campbell, A. M., Nested Primers for PCR (25.9.2020). Department of Biology, Davidson College."
    Comment The logic behind this strategy is that if the wrong locus were amplified by mistake, the probability is very low that it would also be amplified a second time by a second pair of primers.
    PCR nichée | PCR par amorces incluses
    fr
    Definition technique développée pour lutter contre les amplifications parasites consistant à réaliser deux PCR successives en utilisant des couples d'amorces différents Reference "COM-FR d'après le site de Termium Plus®, Ministère Travaux publics et Services gouvernementaux Canada > nested PCR technique, http://www.btb.termiumplus.gc.ca/tpv2alpha/alpha-fra.html?lang=fra&i=1&index=ent&srchtxt=NESTED%20PCR%20TECHNIQUE, [24.9.2015]"