SCIENCE|natural and applied sciences|life sciences|biology
- aistriú eanga Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
- ga
- nick translation
- en
- Sainmhíniú tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in fluorescent in situ hybridization (FISH) or blotting techniques as well as for radiolabeling Tagairt "Wikipedia > Nick translation (24.3.2021)"
- Nóta Nick translation was the first method devised for the in vitro labeling of DNA. During the reaction the DNA to be labeled is nicked by DNase I yielding a free 3′ hydroxyl end. DNA polymerase I then adds a new nucleotide to this end. The 5′–3′ exonuclease activity of the polymerase then moves the “nick” along the strand in the 3′ direction.
- translation de coupure | translation d'entaille | translation de brèche | translation de cassure | translation de césure
- fr
- Sainmhíniú Technique utilisée en biologie moléculaire, permettant de marquer par des radio-isotopes un ADN bicaténaire. L'ADN polymérase 1(d'E. coli) se fixe sur une coupure (un nick) d'un brin, en enlevant les nucléotides à partir de l'extrémité 5'(grâce à son activité exonucléasique 5'3')et les remplace simultanément: il y a copie du brin complémentaire à l'extrémité 3'(grâce à son activité polymérasique 5'3') Tagairt JO F 18.8.87;ADEBIO,p.362