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  1. PRODUCTION, TECHNOLOGY AND RESEARCH|technology and technical regulations|biotechnology

    #620633

    IATE #1142265
    tras-scríobh <i>in vitro</i> Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    in vitro-Transcription
    de
    Sainmhíniú Verfahren, bei dem mRNA zellfrei, d.h.unter Verwendung entsprechend aufbereiteter Zellextrakte, in vitro synthetisiert wird. Tagairt Ibelgaufts H.,Gentechnologie von A bis Z,VCH Verlag
    in vitro transcription
    en
    Sainmhíniú laboratory procedure that allows for template-directed synthesis of RNA molecules of any sequence from short oligonucleotides to those of several kilobases in μg to mg quantities based on engineering of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using the corresponding RNA polymerase Tagairt "Beckert B., Masquida B. (2011) 'Synthesis of RNA by In Vitro Transcription' (3.3.2021). In: Nielsen H. (eds) RNA. Methods in Molecular Biology (Methods and Protocols), vol 703. Humana Press."