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7 dtoradh

  1. INDUSTRY|chemistry

    #602723

    IATE #197286
    leictreafóiréis ghlóthaí Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    Gelelektrophorese
    de
    Sainmhíniú Elektrophorese-Verfahren zur Auftrennung von Gemischen von Proteinen, DNA oder RNA, bei dem ein Gel als Trägermedium genutzt wird Tagairt "COM-DE nach:DocCheck Medical Services GmbH > DocCheck Flexikon > Gelelektrophorese (17.12.2021)"
    gel electrophoresis
    en
    Sainmhíniú method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge Tagairt "Wikipedia > Gel electrophoresis (10.11.2021)"
    Nóta It is used in clinical chemistry to separate proteins by charge or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.
    électrophorèse sur gel
    fr
  2. SCIENCE|natural and applied sciences|physical sciences|chemistry · SCIENCE|natural and applied sciences

    #617614

    IATE #1073516
    leictreafóiréis le glóthach agaróis Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    Agarosegel-Elektrophorese
    de
    Sainmhíniú Ein biochemisches Trennverfahren, bei dem die Wanderung von geladenen Molekülen in einem elektrischen Feld zu deren Trennung ausgenutzt wird. Tagairt Lex.Biochemie;Ibelgaufts H.,Gentechnologie von A bis Z,VCH,Weinheim
    agarose gel electrophoresis
    en
    Sainmhíniú method used in clinical chemistry to separate proteins by charge and or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge Tagairt "Wikipedia > Agarose gel electrophoresis, http://en.wikipedia.org/wiki/Agarose_gel_electrophoresis [18.4.2011]"
    Nóta Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through an agarose matrix. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving. Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins.
    électrophorèse sur gel d'agarose
    fr
  3. SCIENCE|natural and applied sciences|life sciences|biology

    #617615

    IATE #1073517
    leictreafóiréis glóthaí polaicriolaimíde Tagairt "Rialachán Tarmligthe (AE) 2021/1353 lena bhforlíontar Rialachán (AE) 2017/625 ó Pharlaimint na hEorpa agus ón gComhairle a mhéid a bhaineann leis na cásanna agus na coinníollacha faoina bhféadfaidh údaráis inniúla saotharlanna oifigiúla a ainmniú nach gcomhlíonann na coinníollacha maidir leis na modhanna ar fad a úsáideann siad le haghaidh rialuithe oifigiúla nó gníomhaíochtaí oifigiúla eile, CELEX:32021R1353/GA Faomhadh an téarma seo mar chuid de Thionscadal Lex"
    ga
    PAGE Tagairt "Rialachán Tarmligthe (AE) 2021/1353 lena bhforlíontar Rialachán (AE) 2017/625 ó Pharlaimint na hEorpa agus ón gComhairle a mhéid a bhaineann leis na cásanna agus na coinníollacha faoina bhféadfaidh údaráis inniúla saotharlanna oifigiúla a ainmniú nach gcomhlíonann na coinníollacha maidir leis na modhanna ar fad a úsáideann siad le haghaidh rialuithe oifigiúla nó gníomhaíochtaí oifigiúla eile, CELEX:32021R1353/GA Faomhadh an téarma seo mar chuid de Thionscadal Lex"
    ga
    Polyacrylamidgel-Elektrophorese | PAGE | Elektrophorese in Polyacrylamidgel
    de
    Nóta Elektrophorese mit Polyac­ryl­amid­gelen variabler Poren­größe als Trä­ger. In der bio­chemischen Ana­lytik ist PA­GE ei­ne der am häu­figs­ten ver­wen­deten Elektrophorese­tech­niken für die Trennung von Pro­te­inen oder Nukleinsäu­ren im Mikro- bis Nano­gramm­bereich. PA­GE wird ins­besonde­re zur Ana­lyse von Paraproteinen, Pro­te­inbestimmung im Harn und DNA-Sequen­zie­rung ver­wen­det.
    polyacrylamide gel electrophoresis | PAGE
    en
    Sainmhíniú "electrophoretic procedure carried out using a polyacrylamide gel and used to separate a mixed sample of proteins in order to identify and quantify single proteins from the mixture" Tagairt "COM-Terminology Coordination, based on: 'Polyacrylamide Gel Electrophoresis' (1.4.2022), Cleaver Scientific"
    Nóta "There are several types of PAGE technique in use. The most common is SDS-PAGE, which involves using the detergent sodium dodecyl sulfate to denature the proteins and normalise their mass-to-charge ratio (m/z); as a result of denaturation, the proteins separate in the gel based on their mass alone. PAGE without SDS is known as 'native PAGE' because the proteins remain in their native state."
    électrophorèse sur gel de polyacrylamide
    fr
    Sainmhíniú technique d’analyse et de séparation sur du gel de polyacrylamide basée sur les critères de la charge électrique et la taille des molécules très utilisée en immunologie, dans l'étude des protéines et également utilisée pour le séquençage de l'ADN Tagairt "COM-FR d'aprèsTechniques d'analyse - Les techniques électrophorétiques (9.11.2021), Dr BOUTAGHANE.N"
    Nóta Le gel de polyacrylamide est constitué d'acrylamide qui est l'unité de base et de bisacrylamide qui est l'agent portant. En faisant varier les taux de ces 2 substances on obtient différents maillages et donc différentes densités de gel. La polymérisation est réalisée grâce à l'ajout de 2 réactifs: le TEMED (N,N,N',N' tetraméthyl-èthylènediamine) et l'ammonium persulfate qui deviennent des anions hyperactifs en présence de lumière. Plus le pourcentage d’acrylamide est élevé, plus la densité des chaînes est élevée et les mailles du réseau sont serrées. Par conséquent, plus le pourcentage d’acrylamide est élevé, moins les molécules volumineuses peuvent migrer. Donc, plus que la masse moléculaire du composé est élevée, plus que la migration est lente.
  4. SCIENCE|natural and applied sciences|physical sciences|chemistry · AGRICULTURE, FORESTRY AND FISHERIES|agricultural activity|animal health · SOCIAL QUESTIONS|health|medical science · SCIENCE|natural and applied sciences|life sciences

    #620732

    IATE #1147442
    leictreafóiréis ghlóthaí bhíogréimse Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    Pulsfeld-Gel-Elektrophorese | PFGE
    de
    pulsed-field gel electrophoresis | PFGE | pulse-field gel electrophoresis
    en
    Sainmhíniú Gel electrophoresis in which, after electrophoretic migration has begun, the current is briefly stopped and reapplied in a different orientation; allows for the purification of long DNA molecules. Tagairt "http://cancerweb.ncl.ac.uk/cgi-bin/omd?pulsed-field+gel+electrophoresis , (16.04.2008)"
    électrophorèse en champ pulsé
    fr
    Sainmhíniú Technique de séparation des molécules d'ADN qui, coupées en très grands fragments, sont déposées au bord d'une plaque d'agarose gélifié et sont soumises alternativement à deux champs électriques de directions différentes Tagairt Cahier de termes nouveaux,RINT,1991
  5. SCIENCE|natural and applied sciences|life sciences

    #652611

    IATE #3561281
    tíopa bíge Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    PFGE type | Pulsed-field gel electrophoresis typing | PFGE typing | pulse type
    en
    Sainmhíniú "genotype1 determined with pulsed-field gel electrophoresis2 1 genotype [ IATE:1622107] 2 pulsed-field gel electrophoresis [ IATE:1147442 ]" Tagairt "COM-Terminology Coordination, based on:Applied Maths. Pulsed-field gel electrophoresis (PFGE) typing, http://www.applied-maths.com/applications/pulsed-field-gel-electrophoresis-pfge-typing [29.7.2014]"
    Nóta Pulsed-field gel electrophoresis (PFGE) is a highly discriminative molecular typing technique that is used in epidemiological studies worldwide. PFGE is based upon the variable migration of large DNA restriction fragments in an electrical field of alternating polarity. By comparing the fingerprints of any two isolates, one can investigate if they belong to the same strain (i.e. the two isolates are clonal) or if they are genetically unrelated. The implementation of PFGE in international surveillance networks, such as PulseNet, and the availability of standardized protocols for important foodborne pathogens (E. coli, Listeria, Campylobacter, …) have facilitated the adoption of this technique. Although more recent typing methods are available, PFGE is still the gold standard in many national and international surveillance programs.
  6. SCIENCE|natural and applied sciences|life sciences|biology

    #665195

    IATE #3598774
    ais-leictreafóiréis glóthaí polaicriolaimíde Tagairt "Rialachán Tarmligthe (AE) 2021/1353 lena bhforlíontar Rialachán (AE) 2017/625 ó Pharlaimint na hEorpa agus ón gComhairle a mhéid a bhaineann leis na cásanna agus na coinníollacha faoina bhféadfaidh údaráis inniúla saotharlanna oifigiúla a ainmniú nach gcomhlíonann na coinníollacha maidir leis na modhanna ar fad a úsáideann siad le haghaidh rialuithe oifigiúla nó gníomhaíochtaí oifigiúla eile, CELEX:32021R1353/GA ;Faomhadh an téarma seo mar chuid de Thionscadal Lex"
    ga
    R-PAGE Tagairt "Rialachán Tarmligthe (AE) 2021/1353 lena bhforlíontar Rialachán (AE) 2017/625 ó Pharlaimint na hEorpa agus ón gComhairle a mhéid a bhaineann leis na cásanna agus na coinníollacha faoina bhféadfaidh údaráis inniúla saotharlanna oifigiúla a ainmniú nach gcomhlíonann na coinníollacha maidir leis na modhanna ar fad a úsáideann siad le haghaidh rialuithe oifigiúla nó gníomhaíochtaí oifigiúla eile, CELEX:32021R1353/GA ;Faomhadh an téarma seo mar chuid de Thionscadal Lex"
    ga
    Return-Polyacrylamid-Gelelektrophorese | R-PAGE
    de
    return-polyacrylamide gel electrophoresis | R-PAGE
    en
    Sainmhíniú "type of polyacrylamide gel electrophoresis used to identify viroids and viruses with circular RNA in which viroid molecules are subjected to denaturing conditions, thus achieving the separation by slower mobility of denatured viroid molecules" Tagairt "COM-Terminology Coordination, based on: 'R. P. Singh and A. Boucher. Electrophoretic Separation of a Severe from Mild Strains of Potato Spindle Tuber Viroid' (27.4.2021). Phytopathology Vol 77 No 11 1987"
    électrophorèse inverse sur gel de polyacrylamide | électrophorèse sur gel de polyacrylamide «aller-retour» | R-PAGE
    fr
    Sainmhíniú "type d'électrophorèse sur gel de polyacrylamide" Tagairt "COM-FR d'aprèsJaswal, A.S.: «Reuse of buffers in return-polyacrylamide gel electrophoresis tests for the detection of potato spindle tuber viroid», (10.5.2021)American Potato Journal, n.º 66, p. 813–820, 1989."
  7. INDUSTRY|chemistry

    #617613

    IATE #1073514
    leictreafóiréis dhéthoiseach Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    leictreafóiréis ghlóthaí dhéthoiseach Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    leictreafóiréis 2T Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    leictreafóiréis ghlóthaí 2T Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    zweidimensionale Elektrophorese | zweidimensionale Gelelektrophorese | 2D-Elektrophorese | 2D-Gelelektrophorese
    de
    Sainmhíniú Auftrennung komplexer Proteingemische nach zwei unterschiedlichen, voneinander unabhängigen physikochemischen Parametern, zunächst nach Ladungen mit der isoelektrischen Fokussierung (IEF) und anschließend nach Molekülgrößen mit der SDS-Elektrophorese Tagairt "COM-DE nach:Reiner Westermeier: „Elektrophorese, zweidimensionale“. In: Axel M. Gressner / Torsten Arndt (Hrsg.): Lexikon der Medizinischen Laboratoriumsdiagnostik. Springer: Berlin, Heidelberg, 2019, S. 771-773. DOI: 10.1007/978-3-662-48986-4_990 (21.12.2021)"
    Nóta Um komplexe Proteingemische mit hoher Auflösung und guter Reproduzierbarkeit auftrennen zu können, werden denaturierende Bedingungen angewandt. Das bedeutet, dass die Probe mit 8 mol/L Harnstoff, einem Reduktionsmittel, einem zwitterionischen Detergenz und einem Trägerampholytengemisch versetzt oder extrahiert wird.Genügend hohe Auflösung für komplexe Proteingemische erhält man nur in Gelen mit großen Trenndistanzen, z. B. 20 × 20 cm.
    two-dimensional electrophoresis | two-dimensional gel electrophoresis | 2-D electrophoresis | 2D gel electrophoresis | 2DE
    en
    Sainmhíniú protein separation technique by molecular charge and molecular size, which combines isoelectric focusing in one dimension and gel electrophoresis in the second dimension, resulting in better separation of proteins as the two parameters are unrelated Tagairt "COM-Terminology Coordination, based on: - Nicole Kresge, Robert D. Simoni, Robert L. Hill. 'The Development of Two-dimensional Electrophoresis by Patrick H. O'Farrell' (10.11.2021). JBC Volume 281, ISSUE 32, e26-e27, August 2006- Smith R. 'Two-dimensional electrophoresis: an overview' (10.11.2021). Methods Mol Biol. 2009;519:1-16. doi: 10.1007/978-1-59745-281-6_1. PMID: 19381573."
    Nóta There are many variants depending on the gel used.
    électrophorèse sur gel bidimensionnelle | electrophorèse bidimensionnelle | électrophorèse sur gel 2D
    fr
    Sainmhíniú Méthode combinant deux électrophorèses, une séparation selon la charge électrique des protéines, suivie d'une séparation en fonction de leurs poids moléculaire Tagairt Biofutur 5/83,p.7