SCIENCE|natural and applied sciences|life sciences
- RT-PCR aonchéime fíor-ama Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
- ga
- real time single-step RT-PCR | one-step real-time RT-PCR
- en
- Sainmhíniú "qPCR1 technique, in which reverse transcription (RT)2 and qPCR steps are both conducted in the same reaction well, reducing the number of sample manipulations required and time to result1qPCR [ IATE:3541735 ]2reverse transcription [ IATE:1464555 ]" Tagairt "COM-Terminology Coordination, based on:Life Technologies. One-Step Real-Time RT-PCR Master Mixes, https://www.lifetechnologies.com/lu/en/home/life-science/pcr/real-time-pcr/real-time-pcr-reagents/one-step-real-time-rt-master-mix.html [17.11.2015]"
- Nóta "Unlike two-step strategies, all of the cDNA1 is used in the PCR, so a cDNA archive is not available. One-step RT-PCR is most commonly used to process many samples at a time, for one or a few targets per sample. Examples include virus detection and quantification, high-throughput gene expression screening, and simple RNA quantification experiments.1cDNA [ IATE:1073342 ]See also:- reverse transcription [ IATE:1464555 ]- polymerase chain reaction [ IATE:1431892 ]- real-time PCR, qPCR [ IATE:3541735 ]"