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5 thoradh

  1. SCIENCE|natural and applied sciences|life sciences

    #574315

    IATE #3510243
    RT-PCR aonchéime fíor-ama Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    one-step real-time RT-PCR | real time single-step RT-PCR
    en
    Sainmhíniú qPCR1 technique, in which reverse transcription (RT)2 and qPCR steps are both conducted in the same reaction well, reducing the number of sample manipulations required and time to result1qPCR [ IATE:3541735 ]2reverse transcription [ IATE:1464555 ] Tagairt COM-Terminology Coordination, based on:Life Technologies. One-Step Real-Time RT-PCR Master Mixes, https://www.lifetechnologies.com/lu/en/home/life-science/pcr/real-time-pcr/real-time-pcr-reagents/one-step-real-time-rt-master-mix.html [17.11.2015]
    Nóta Unlike two-step strategies, all of the cDNA1 is used in the PCR, so a cDNA archive is not available. One-step RT-PCR is most commonly used to process many samples at a time, for one or a few targets per sample. Examples include virus detection and quantification, high-throughput gene expression screening, and simple RNA quantification experiments.1cDNA [ IATE:1073342 ]See also:- reverse transcription [ IATE:1464555 ]- polymerase chain reaction [ IATE:1431892 ]- real-time PCR, qPCR [ IATE:3541735 ]
    RT-PCR en une étape «en temps réel»
    fr
  2. SCIENCE|natural and applied sciences|life sciences

    #586968

    IATE #3563585
    ISP-CT dhá chéim Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    two-step RT-PCR
    en
    Sainmhíniú RT-PCR1 done in two steps, first with the reverse transcription and then the PCR21 RT-PCR [ IATE:1904611 ]2 PCR [ IATE:1431892 ] Tagairt MIT. MITOPENCOURSEWARE. RT-PCR: Two-Step Protocol, http://ocw.mit.edu/courses/biology/7-16-experimental-molecular-biology-biotechnology-ii-spring-2005/labs/rt_pcr_2step.pdf [17.11.2015]
  3. SCIENCE|natural and applied sciences|life sciences|biology|genetics

    #574269

    IATE #3510195
    ISP-CT neadaithe Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    imoibriú slabhrúil polaiméaráise neadaithe cúl-trascrioptáise Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    mehrstufige RT-PCR
    de
    nested set RT-PCR | nested RT-PCR | reverse-transcription nested polymerase chain reaction
    en
    Sainmhíniú technique based on RT-PCR1 where a sample of the product from a primary RT-PCR is taken and used as a template for a secondary round of PCR2 amplification3 in which a different set of primers4 (called "nested" primers because they are internal to the primers used in the primary PCR) is employed to avoid further amplification of primer-dimer5 artifacts or nonspecific products generated in the primary PCR Tagairt COM-Terminology Coordination, based on: Goode T. et al. (2002) Nested RT-PCR (12.4.2019). In: O’Connell J. (eds) RT-PCR Protocols. Methods in Molecular Biology, vol 193. Humana Press
    Nóta 1 RT-PCR: 19046112 PCR: IATE:1431892<;;;; amplification: 1473998 4 primers:10733775 primer-dimer artefacts: 3580676
  4. SCIENCE|natural and applied sciences|life sciences|biology|genetics

    #586964

    IATE #3563577
    imoibriú slabhrúil polaiméaráise cúl-trascrioptáise cainníochtúil fíor-ama Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    Echtzeit-Reverse-Transkriptase-Polymerase-Kettenreaktion | quantitative Echtzeit-RT-PCR
    de
    real-time reverse-transcription polymerase chain reaction | RT-(q)PCR | real-time RT-PCR | RT-qPCR | quantitative RT-PCR | real-time quantitative reverse transcription polymerase chain reaction | real-time reverse transcriptase polymerase chain reaction | quantitative real-time RT-PCR | real-time reverse transcription PCR | qRT-PCR
    en
    Sainmhíniú in vitro1method for enzymatically amplifying defined sequences of RNA2 1 RNA (related) [ IATE:1073359 ] 2 in vitro (related) [ IATE:1364825 ] Tagairt Jelena Brkic. Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Analysis. BIOL5081. York University, http://www.yorku.ca/shore/Grad%20stats/qRT%20PCR%20posting.pptx [18.12.2014]
    réaction en chaîne par polymérase en temps réel après transcription inverse | qRT-PCR | RT-PCR quantitative
    fr
    Sainmhíniú technique ayant pour but de quantifier un type d’ARN initialement présent dans un échantillon par la réalisation d'une PCR (réaction en chaîne par polymérase) à partir d’un échantillon d’ARN d'abord rétrotranscrit grâce à une enzyme appelée transcriptase inverse, qui permet la synthèse de l’ADN complémentaire (ADNc) utilisé pour la PCR Tagairt COM-FR d'après le site de Futura-sciences > santé > dictionnaire > RT-PCR, http://www.futura-sciences.com/magazines/sante/infos/dico/d/medecine-rt-pcr-13491/ [23.9.2015]
  5. SCIENCE|natural and applied sciences|life sciences|biology|genetics

    #568567

    IATE #1904611
    imoibriú slabhrúil polaiméaráise cúl-trascrioptáise Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    ISP cúl-trascrioptáise Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    Umkehrtranskriptase-PCR | Reverse-Transkriptase-Polymerase-Kettenreaktion | RT-PCR
    de
    reverse transcriptase-PCR | reverse transcriptase polymerase chain reaction | reverse transcription-polymerase chain reaction | RT-PCR
    en
    Sainmhíniú laboratory technique combining reverse transcription [ 1464555 ] of RNA into DNA and amplification of specific DNA targets using polymerase chain reaction (PCR) [ 1431892 ] Tagairt - 'Reverse transcription polymerase chain reaction' (21.5.2019), Wikipedia.
    transcription inverse couplée à une réaction d’amplification en chaîne par polymérase
    fr