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2 thoradh

  1. SCIENCE|natural and applied sciences|life sciences|biology|genetics

    #681278

    IATE #1904614
    imoibriú slabhrúil polaiméaráise neadaithe Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    ISP neadaithe Tagairt Téarmeolaithe COM-GA
    ga
    verschachtelte PCR | verschachtelte Polymerase-Kettenreaktion
    de
    nested polymerase chain reaction | nested PCR | nPCR
    en
    Sainmhíniú "method of polymerase chain reaction (PCR) which uses two pairs of PCR primers for a single locus, the first pair amplifying the locus as seen in any PCR experiment, while the second pair of primers (nested primers) binds within the first PCR product and produces a second PCR product that will be shorter than the first one" Tagairt "COM-Terminology Coordination, based on:Campbell, A. M., Nested Primers for PCR (25.9.2020). Department of Biology, Davidson College."
    Nóta The logic behind this strategy is that if the wrong locus were amplified by mistake, the probability is very low that it would also be amplified a second time by a second pair of primers.
    PCR nichée | PCR par amorces incluses
    fr
    Sainmhíniú technique développée pour lutter contre les amplifications parasites consistant à réaliser deux PCR successives en utilisant des couples d'amorces différents Tagairt "COM-FR d'après le site de Termium Plus®, Ministère Travaux publics et Services gouvernementaux Canada > nested PCR technique, http://www.btb.termiumplus.gc.ca/tpv2alpha/alpha-fra.html?lang=fra&i=1&index=ent&srchtxt=NESTED%20PCR%20TECHNIQUE, [24.9.2015]"
  2. SCIENCE|natural and applied sciences|life sciences|biology|genetics

    #682222

    IATE #3510195
    ISP-CT neadaithe Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    imoibriú slabhrúil polaiméaráise neadaithe cúl-trascrioptáise Tagairt Faomhadh an téarma seo mar chuid de Thionscadal Lex
    ga
    mehrstufige RT-PCR
    de
    nested RT-PCR | nested set RT-PCR | reverse-transcription nested polymerase chain reaction
    en
    Sainmhíniú "technique based on RT-PCR1 where a sample of the product from a primary RT-PCR is taken and used as a template for a secondary round of PCR2 amplification3 in which a different set of primers4 (called ""nested"" primers because they are internal to the primers used in the primary PCR) is employed to avoid further amplification of primer-dimer5 artifacts or nonspecific products generated in the primary PCR" Tagairt "COM-Terminology Coordination, based on: Goode T. et al. (2002) Nested RT-PCR (12.4.2019). In: O’Connell J. (eds) RT-PCR Protocols. Methods in Molecular Biology, vol 193. Humana Press"
    Nóta "1 RT-PCR: 19046112 PCR: IATE:1431892<;;;;;;;;; amplification: 1473998 4 primers:10733775 primer-dimer artefacts: 3580676 "